【摘要】 研究了偶氮胂Ⅲ-Ba(Ⅱ)配合物与牛血清白蛋白(BSA)三体系复合物的吸收光谱,建立了一种新的蛋白质定量分析方法。研究发现,在表面活性剂阿拉伯树胶(gum arobic)存在下的酸性溶液中,偶氮胂Ⅲ-Ba(Ⅱ)配合物与BSA发生反应形成三元复合物,引起吸收光谱发生红移,吸收峰明显增高。摩尔吸光系数为ε611=1.01×106L.mol-1.cm-1,BSA的质量浓度在0~67 mg/L范围内服从比尔定律。用摩尔比法研究了此配合物与BSA的最大结合数为51。该法灵敏度高,选择性好,用于实际血清样品总蛋白的测定,与双缩脲法相关性良好。更多还原

【Abstract】 A novel method based on the binding reaction of arsenazoⅢ-barium(Ⅱ) with bovine serum albumin(BSA) was developed for the determination of protein in human serum.The optimum reaction conditions and the influencing factors were studied.In the presence of gum arobic(surfactant),arsenazo Ⅲ-barium(Ⅱ) in acid solution could combine with BSA to form a ternary complex leading to a redshift of absorption spectrum and significant increase of absorption peak intensity.The molar absorption coefficient was 1.01×106 L·mol-1·cm-1.The calibration curve for BSA was linear up to 67 mg/L.The binding number for the complex and BSA determined by molar ratio method was 51.The method has been applied to the determination of protein in human serum.The results obtained agreed quite well with those obtained by Biuret method.更多还原