【摘要】 目的:探讨血清沙眼衣原体(CT)及其热休克蛋白60(C-HSP60)抗体反应与输卵管性不孕(TFI)的关系。方法:检测了60例TFI患者(TFI组)和30例正常生育妇女(对照组)血清CT抗体。用基因工程的方法重组C-HSP60,以纯化后的C-HSP60蛋白为抗原,采用酶联免疫吸附法(ELISA),检测二组对象血清C-HSP60抗体水平。结果:TFI组和对照组血清沙眼衣原体IgG抗体阳性率分别为73.22%和43.33%,组间有显著性差异(P<0.05);TFI组C-HSP60IgG抗体阳性率为46.67%,高于对照组阳性率6.67%,差异有统计学意义(P<0.05)。TFI组中28例C-HSP60IgG抗体阳性者与32例阴性者相比,阳性者的输卵管损害程度高于阴性者(P<0.01),而阴性者的盆腔粘连程度则较阳性组严重(P<0.01)。结论:女性生殖道沙眼衣原体感染是导致TFI的重要原因,C-HSP60与其发病机理有非常密切的关系。更多还原

【Abstract】 Objective: To investigate the prevalence of antibody to Chlamydia trachomatis and chlamydial heat shock protein 60 (C-HSP60) in women with tubal factor infertility(TFI). Methods: Sixty women with tubal factor infertility (TFI group) and 30 women without tubal pathology (control group) were investigated to control study. An ELISA using purified recombinant C-HSP60 was developed to detect antibodies to Chlamydia trachomatis and C-HSP60 in serologic samples. Results: The positive rate of IgG antibody to Chlamydia trachomatis was 73.22% of 60 women in TFI group compared with 43.33% of 30 women in control group (P<0.05); C-HSP60 IgG positive rate was significantly higher in TFI group than in control group (46.67% vs 6.67%,P<0.05). Among the TFI group,the cases of antibody to C-HSP60 positive women were presented more tubal damage and less pelvic adhesions degree than C-HSP60 negative women (P<0.05). Conclusion: There is a strong association between antibody response to C-HSP60 and the development of Chlamydia trachomatis associated TFI. So C-HSP60 plays an important role in the tubal factor infertility.更多还原