【摘要】 目的研究转化生长因子(TGF)-β1反义寡核苷酸(ASON)对人胚胎性横纹肌肉瘤细胞系RD增殖的影响。方法采用反义寡核苷酸技术,以阳离子脂质体为载体,将人工合成的TGF-β1 ASON转染RD细胞,阻碍内源性TGF-β1/Smad信号的转导,以未转染的RD细胞组作对照。免疫荧光技术检测RD细胞TGF-β1蛋白表达水平;四甲基偶氮唑盐实验(MTT)检测RD细胞的增殖情况;流式细胞术(FCM)检测细胞周期。结果转染4 d后RD细胞TGF-β1蛋白表达量较转入2 d时明显减少,与对照组比较,差异有统计学意义(P<0.05);RD细胞活力明显降低,合成期(S期)细胞减少。结论TGF-β1 ASON可有效阻碍TGF-β1/Smad信号通路,在一定程度上可促进RD细胞退出细胞周期,并抑制RD细胞增殖。更多还原

【Abstract】 Objective To study the effects of transforming growth factor (TGF)-β1 antisense oligonucleotides(ASON) on proliferation of human rhabdomyosarcoma cell line RD.Methods Artificial- synthesized TGF-β1 ASON was transfected into RD cells by cation liposome vector to block endogenous TGF-β/Smad signal transduction,and RD cells without transfection served as the control group.The TGF-β1 protein expression of RD cells was inspected by immunofluorescent.The proliferation status of RD after TGF-β1 ASON-transfection was examined by MTT.The cell cycle was detected by FCM.Re- sults The TGF-β1 protein expression of RD cells transferred for 4 days was decreased more than that of RD cells transferred for 2 days with significant difference (P<0.05) compared to the control group. The viability of RD cells in test group decreased obviously,and the S cells decreased.Conclusion The TGF-β1/Smad signal transduction pathway can be effectively blocked by the TGF-β1 ASON.TGF-β1 ASON could induce RD cells exit cell cycle,and inhibit the proliferation of RD cells.更多还原