【摘要】 目的探讨术前区域射频热疗同步靶动脉热灌注化疗对胃癌淋巴结转移灶癌细胞凋亡的影响。方法对40例胃癌伴淋巴结转移患者行胃癌根治性切除术,根据术前是否行区域射频热疗同步靶动脉热灌注化疗分为治疗组和对照组,每组20例,采用免疫组化ABC法检测淋巴转移灶p53、bcl-2及CD95基因的表达,应用原位末端脱氧核苷转移酶标记法(TUNEL)检测细胞凋亡情况。结果治疗组淋巴转移灶p53和CD95表达较对照组明显增强,差异有统计学意义(P<0.05),并与细胞凋亡呈正相关;bcl-2表达则减低,差异有统计学意义(P<0.05),与细胞凋亡呈负相关。结论胃癌术前行区域射频热疗同步靶动脉热灌注化疗,可能通过p53、bcl-2及CD95介导使转移淋巴结癌细胞凋亡增加,有助于提高根治性手术的疗效。更多还原

【Abstract】 Objective To study the effect of preoperative region radio-frequency thermotherapy synchronizating with target arterial thermochemotherapy on apoptosis of lymph node metastasis of gastric cancer.Methods Forty patients with gastric cancer and lymph node metastasis underwent curative resection,among which there were 20 patients who received the preoperative gastric arterial chemoembolization,and they constituted the treatment group.The rest of the patients were included in the control group.The expressions of p53,CD95 and bcl-2 was examined by immunohistochemistry and apoptosis in the lymph node metastasis was examined by in situ terminal transferase mediated dUTP nick end labeling(TUNEL).Results The expression intensity of p53 and CD95 in lymph node metastasis of treatment group increased more significantly than that in the control group,whereas the expression intensity of bcl-2 decreased in the treatment group.There was a significantly positive correlation between the expressions of p53 and CD95 and the apoptosis.Conclusion Preoperative region radio-frequency thermotherapy and synchronizating with target arterial thermochemotherapy may affect the expressions of p53,CD95 and bcl-2 and may induce the apoptosis of lymph node metastasis.It may be helpful to improve the effect of curative resection of gastric cancer.更多还原