【摘要】 目的研究参附注射液对缺氧缺血性脑损伤(HIBD)新生大鼠海马神经元凋亡的影响。方法实验分成两部分:1.流式细胞术检测缺氧缺血(HI)前2 h、HI后2、12、24 h,3、7、14和28 d各时间点新生大鼠海马CA1区神经元凋亡率;2.免疫组织化学测HI前2 h、HI后2、12、24 h,3、7、14和28 d各时间点新生大鼠海马CA1区神经元Bcl-2、Bax蛋白表达。建立新生大鼠HIBD模型。每组实验随机分为4组:假手术组(S),9 g/L盐水对照组(C),参附预处理组(P),参附治疗组(SF)。结果SF和P组海马神经元凋亡情况明显低于C组(P<0.01,<0.05),且Bcl-2表达显著增多(P<0.05,<0.01),而Bax表达明显下调(P<0.05,<0.01)。结论参附注射液可明显上调新生大鼠HIBD后海马神经元Bcl-2蛋白表达,下调Bax蛋白表达,减少HIBD新生大鼠海马神经元凋亡发生。更多还原

【Abstract】 Objective To study the effect of shenfu injection on the neuron apoptosis in hippocampal CA1 region of newborn with hypoxic-ischemic brain damage(HIBD).Methods The experiment included 2 parts.One was to measure the apoptosis rate by the flow cytometry,and the other was to investigate the expression of Bcl-2 and Bax of neurons in left hippocampal CA1 region.Models of postnatal 7-day Sprague-Dawley(SD)rats with HIBD were established,and were equally divided into 4 groups:sham operation(group S),control group(group C),shenfu injection pretreatment(group P),and shenfu injection treatment(group SF).The neuron apoptosis rate in hippocampal CA1 region in every group was measured at 2 h before and 2,12,24 h,3,7,14 and 28 d after hypoxic ischemic(HI) insult.The expressions of Bcl-2 and Bax were performed by immunohisochemistry.Results The apoptosis of neuron in hippocampal CA1 region in group P and group SF after HI insult was significantly less than that of group C(P<0.01,<0.05).The expression of Bcl-2 was markedly higher than that in group C(P<0.05,<0.01)and the expression of Bax was markedly down-regulated(P<0.05,<0.01).Conclusion Shenfu injection effectively prevents apoptosis of hippocampus neurons from HI insult by up-regulation of the anti-apoptotic gene Bcl-2 and down-regulation of the pro-apoptotic gene Bax.更多还原