【摘要】 目的:研究新型医用钛合金Ti-24Nb-4Zr-7.9Sn(TNZS)经过阳极氧化(AD)技术处理后体外诱导活性及对人成骨样MG63细胞早期附着的影响。方法:采用阳极氧化处理TNZS合金,对其进行模拟体液(SBF)浸泡实验,分析其表面元素成分及相结构的变化;并将人成骨样MG63细胞接种于Ti6Al4V、TNZS、AD-TNZS表面,观察细胞的早期附着及形态学变化。结果:AD-TNZS在SBF中浸泡6d后,材料表面有羟基磷灰石形成。在MG63成骨样细胞接种60、120min时,人成骨样MG63细胞在AD-TNZS表面的附着率均明显高于Ti6Al4V和TNZS表面附着率(P<0.05),并表现出良好的形态。结论:阳极氧化后的新型医用钛合金在模拟体液中可诱导羟基磷灰石形成;对成骨细胞的早期附着有一定的促进作用。更多还原

【Abstract】 Objective: To study the vitro bioactivity of the Ti-24Nb-4Zr-7.9Sn (TNZS) disks modified by anodic oxidation(AD) and its effects on adhesion of human osteoblast-like MG63 cells. Methods:The TNZS titanium alloy disks were treated with anodic oxidation and the bioactivity was assessed by investigating the formation of apatite on the film surface after soaking in simulated body fluids. The surface composition of the specimens after immersion was evaluated by EDX and X-ray diffraction. MG63 osteoblast-like cells were seeded on the Ti6Al4V,TNZS,AD-TNZS disks, and then the number of attached cells were counted and the cell morphology was examined at each given period. Results:The apatite can form on the treated TNZS disks after soaking in SBF for 6 days. The ratio of attached MG63 on AD-TNZS disks after 60 or 120 min seeding were significant higher than that on other samples and the cells on AD-TNZS disks spread better. Conclusion:The TNZS alloy modified by anodic oxidation can induce apatite formation in SBF and increase the early attachment of osteoblasts.更多还原