【摘要】 采用正交试验优化桦褐孔菌子实体多糖的超声提取工艺条件,并对提取多糖的抗肿瘤活性进行研究。结果表明,桦褐孔菌多糖超声提取的最佳工艺条件为超声时间70min,温度80℃,频率20kHz,加水量12倍水,其多糖在体外对肝癌HEPG2细胞株具有明显的抑制作用,并可诱导肿瘤细胞凋亡,体内实验发现多糖可显著抑制小鼠S180肉瘤的生长,并具有增强免疫作用。更多还原

【Abstract】 Optimal conditions for extraction of polysaccharide from Inonotus obliquus fruit bodies(IOP)were determined as follows:extraction time,70 min;temperature,80 ℃;ratio of aqueous solvent to fruit body,12∶1;and ultrasound frequency,20 kHz.IOP at a concentration of 1.44 μg/mL inhibited cancer cell growth by~65% as measured using a tetrazolium-based(MTT)assay to evaluate the anti-proliferative effect of the polysaccharide on hepatocarcinoma HEPG2 cells.A 27% and 35% reduction in tumor weight was also observed in S-180 tumor-bearing mice administered low(50 mL/kg·d,8 μg/mL)and high(100 mL/kg·d,16 μg/mL)doses of IOP,respectively.更多还原