【摘要】 目的探讨Bcl-2的表达与甲状腺癌的发生、发展及预后的关系。方法应用免疫组化LSAB法,以单克隆抗体鼠抗人Bcl-2,标记65例甲状腺癌、50例甲状腺腺瘤、30例癌旁甲状腺组织和20例正常甲状腺组织。观察不同甲状腺组织中Bcl-2的表达,并比较其阳性率。结果Bcl-2阳性反应见于甲状腺癌、甲状腺腺瘤及癌旁甲状腺组织。在甲状腺癌组织中Bcl-2阳性率为44.6%,高于甲状腺腺瘤组织(22.0%)(P=0.012)、癌旁甲状腺组织(16.7%)(P=0.008)和正常甲状腺组织(0)(P=0.0000)。甲状腺未分化癌和髓样癌组织中Bcl-2的阳性率明显增高。存在淋巴结转移和临床Ⅲ、Ⅳ期病例中,Bcl-2的阳性率明显增高。结论Bcl-2过量表达可能与甲状腺肿瘤的发生有关,癌组织中Bcl-2的表达可作为甲状腺癌预后的参考指标。更多还原

【Abstract】 Objective To investigate the expression of protooncogene Bcl-2 in thyroid tumors and its relationship to the oncogenesis,development and prognosis of the tumor.Methods The Expression of Bcl-2 was analyzed by immunohistochemical LSAB method in the tissues from 65 thyroid carcinomas,50 thyroid adenomas,30 thyroid tissues adjacent to cancer and 20 normal thyroid tissues.The positive expression rates in the different thyroid tissues were compared.Results Bcl-2 was expressed in 44.6% of the throid carcinoma,22.0% of the thyroid adenoma,16.7% of the thyroid tissues adjacent to cancer and non in normal thyroid tissues.The positive expression rate of Bcl-2 in the thyroid carcinoma was significantly higher than that in the thyroid adenoma(P= 0.0120),thyroid tissues adjacent to cancer (P= 0.0080)and normal tissues (P= 0.0000).In thyroid carcinoma,undifferentiated carcinoma,medullary carcinoma,carcinoma with positive lymph nodes,and tumors with stage Ⅲ and Ⅳ disease had significantly higher expression of Bcl-2.Conclusion The overexpression of Bcl-2 may be related to the oncogenesis and development of thyroid tumor.Bcl-2 might be a possible prognostic factor of thyroid cancer.更多还原