【摘要】 目的观察阿司匹林(aspirin/acetylsalicylic acid,ASA)预处理对大鼠局灶性脑缺血再灌注损伤(I/R)的保护作用,并对其作用机制进行探讨。方法健康雄性SD大鼠,随机分为假手术组、模型+溶酶组、ASA 100 mg/kg预处理组,以线栓法制作大鼠大脑中动脉缺血再灌注模型,24 h后进行神经功能评分并断头取脑分别测定脑梗死体积、脑组织匀浆诱导型一氧化氮合酶(iNOS)活性和一氧化氮(NO)含量。结果与模型+溶酶组相比,ASA预处理组的脑梗死体积明显减小(P<0.05),神经功能缺损评分获不同程度改善(P<0.05),脑组织中iNOS活性、NO含量明显下降(P<0.01)。结论ASA对大鼠脑缺血再灌注损伤有一定保护作用,其作用机制与抑制脑组织中iNOS活性,降低NO含量有关。更多还原

【Abstract】 Objective To explore neuroprotective mechanism of aspirin preconditioning on cerebral ischemia reperfusion injury in Sprague-Dawley rats.Methods Adult male SD rats were randomly divided into the sham-operation group,ischemia control group,aspirin preconditioning group.The middle cerebral artery occlusion/reperfusion(MCAO/R) model was made by the suture method.After 24 h the animals were neurologically assessed on a 5-point scale,and then beheaded.The volume of infarction was measured by TTC staining,and nitric oxide(NO) and inducible nitric oxide synthase(iNOS) activity in brain tissue homogenate were measured.Results Compared with ischemia control group,the volume of infarction significantly reduced in aspirin preconditioning group(P<0.05),the neurological scores improved(P<0.05),and NO levels and iNOS activities significantly decreased(P<0.01).Conclusion Aspirin preconditioning has neuroprotective effect on cerebral ischemia reperfusion injury,which may be related inhibition of iNOS activity and decrease of NO level.更多还原