【摘要】 目的:探讨胡杨愈伤组织的耐盐性与生理生化指标的关系。方法:不同浓度NaCl胁迫下,对胡杨愈伤组织的丙二醛(MDA)含量、过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性以及酯酶同工酶、过氧化物酶同工酶、超氧化物歧化酶同工酶酶谱进行研究。结果:随着盐浓度的增加,丙二醛(MDA)含量逐渐升高;从三种保护酶活性来看,过氧化物酶(POD)和过氧化氢酶(CAT)活性均呈先上升后下降的趋势,在70mmol/LNaCl胁迫下达到最高值,POD、CAT酶活分别为2748u/g.min和152u/g.min,分别比对照高104%和72%;超氧化物歧化酶(SOD)活性则呈先降低后稍上升的趋势,总体均比对照低。过氧化物酶同工酶的第2、3条酶带在70mmol/LNaCl胁迫下颜色最深,且酶带较宽;超氧化物歧化酶同工酶的第1条酶带仅在未经盐胁迫时存在,说明该酶带所代表的酶对盐很敏感;第4条酶带随盐胁迫浓度加大先消失后出现,可能是愈伤组织逐渐适应盐胁迫的结果。结论:经盐胁迫的胡杨愈伤组织中,POD和CAT酶主要起保护作用;结合同浓度盐胁迫下愈伤组织的形态观察,总体上看,胡杨愈伤组织能较好地耐受70mmol/LNaCl。更多还原

【Abstract】 It was to search the relationship between calli of Populus euphratica on the salt tolerance and its physiological and biochemical indexes.Methods:Effects of NaCl stress on the malondialdenyde(MDA)content;the activities of peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD);the zymogram of esterase isozyme,peroxidase isozyme and superoxide dismutase isozyme of calli of Populus euphratica were investigated.Results:The malondialdenyde(MDA)content increased gradually with NaCl stress ;according to three enzymes activities,the activities of peroxidase(POD)and catalase(CAT)increased firstly,then decreased as the stress content increased,and reached the peak-2 748u/g·min and 152u/g.min respectively under 70 mmol/L NaCl,which were 104% and 72% higher than contrast respectively.But superoxide dismutase(SOD)activities decreased firstly and then increased slightly,which was lower than the contrast on the whole level.The second and the third bands of POD had the deepest colour under 70mmol/L NaCl and were wider;the first band of SOD appeared only without salt stress,which indicated its relevant enzyme was sensitive to salt;the forth one firstly vanished and then appeared following to NaCl’s increase,which is likely because the calli adapted salt stress gradually.Conclusion:In the mass,POD and CAT mainly played protective roles in calli under salt stress.Combined morphological observation of calli under the same content,the calli could bear 70mmol/L NaCl preferably.更多还原