【摘要】 目的:探讨基质金属蛋白酶类与胶质细胞瘤浸润性生长之间的关系及其在胶质瘤复发中的作用。以及基质金属蛋白酶及其抑制因子(MMP-2、TIMP-2)的阳性表达与胶质细胞瘤病理分级及预后的关系。方法:应用免疫组织化学染色法(SP法)检测48例人脑原发和复发胶质细胞瘤组织中MMP-2、TIMP-2的表达。结果:Ⅲ、Ⅳ级和复发胶质细胞瘤中的MMP-2蛋白的表达显著高于Ⅰ、Ⅱ级(P<0.05);Ⅰ、Ⅱ级胶质细胞瘤中的TIMP-2蛋白的表达显著高于复发胶质细胞瘤(P<0.05)而与Ⅲ、Ⅳ级细胞瘤无显著性差异(P>0.05);Ⅲ、Ⅳ级和复发胶质细胞瘤中的MMP-2,TIMP-2蛋白的表达无显著差异(P>0.05);同级别胶质细胞瘤中MMP-2、TIMP-2的表达之间无明显相关性(P>0.05);正常脑组织中无表达。结论:MMP-2、TIMP-2的表达与胶质细胞瘤的恶性程度有关,低级别中,MMP-2与TIMP-2成正相关;MMP-2的高表达,TIMP-2的低表达与胶质细胞瘤的侵袭有关,MMP-2、TIMP-2的表达水平有可能成为判定胶质细胞瘤恶性程度、侵袭能力和预后的诊断指标,而且TIMP-2可能更加敏感。更多还原

【Abstract】 Objectives:To study the relationship between matrix metalloproteinases and their inhibitors and the invasion and metastasis of human glioma,as well as the relationship between the expression of MMP-2 and TIMP-2 in human primary and recrudes- cent glioma and the grade of malignancy.Methods:Expression of MMP-2 and TIMP-2 were detected in 60 cases of glioma by immuno- histochemical staining.Results:Expression of MMP-2 in gradeⅠandⅡglioma were significantly lower than that in gradeⅢandⅣglioma and the recrudescent glioma,while expression of TIMP-2 in recrudescent glioma was significantly lower than that in gradeⅠandⅡglioma.The expression of MMP-2 and TIMP-2 in normal brain was negative.There was no significant difference between expression of MMP-2 and TIMP-2 in the same grade glioma.Conclusions:Expression of MMP-2 and TIMP-2 is positively related to the malignancy and invasion of glioma.It is also suggested that expression of MMP-2 and TIMP-2 in human glioma may become an assessable factor for identifying the malignancy and invasion of glioma and evaluating the results of therapy.更多还原