【摘要】 采用微管吮吸技术测定了大鼠肝细胞的黏弹性,进一步研究了丙烯酰胺(acrylamide)和细胞松弛素D(cytochalasin D,CD)以及两者混合作用后对于细胞黏弹性的影响。结果表明:在用CD、丙烯酰胺以及CD、丙烯酰胺混合处理细胞后发现细胞的黏弹性系数均明显下降。尤其是对K1的影响最大中间纤维对于黏弹性系数K1的影响要大于微丝对K1影响。而微丝于黏弹性系数K2的影响要大于中间纤维对K1的影响。另外在丙烯酰胺和细胞松弛素D两者混合作用后细胞的黏弹性系数均比两者单独使用时呈显著下降的趋势。提示中间纤维、微丝在维持细胞黏弹性这一生物力学特性方面均起着一定的作用。另外从以丙烯酰胺组和细胞松弛素D组为照组的数据中发现:丙烯酰胺和细胞松弛素D两者混合作用后细胞的黏弹性系数K1或K2也存在一定程度的降低。中间纤维和微丝可能对正常肝细胞黏弹性有一定之间的交互影响的作用。更多还原

【Abstract】 In this study,we adopted micropipe technology to measure the viscoelasticity of liver cell fromrat,and then re- searched the influence of acrylamide,cytochalasin D(CD),and the mixture of the two on viscoelasticity of cell.The results showed that after the treatment of CD,acrylamide and the mixture the two,the viscoelasticity of cell decreased apparently.It is indicated that the in- fluence of microtubules on viscoelasticity coefficient K 1 is more obvious than microfilaments,the influence of intermediate filament(IF) on viscoelasticity coefficient K1 is more obvious than microfilaments,but the influence of microfilaments on viscoelasticity coefficient K2 is more obvious than intermediate filament IF on viscoelasticity coefficient K1.After treatment with the mixture of acrylamide and cytochalasin D,the viscoelasticity coefficient of the cell decreased more apparently than the treatment with single drug.It reveals that the intermediate filament(IF)and the microfilaments took the effect on keeping the viscoelasticity of cell.In addition,after treatment with the mixture of both drugs the viscoelasticity coefficient K1 or K2 decreased.The interaction of intermediate filament (iF)and microfila- ments took the effect on viscoelasticity of hepatic cell.更多还原