【摘要】 对26尾三倍体湘云鲫的线粒体tRNA-Thr基因、tRNA-Pro基因和部分控制区的核苷酸序列进行了测定,获得26条长度为837—839 bp的同源基因序列,共发现65个多态性核苷酸变异位点,多态位点比例为0.077,定义了8种单元型。在湘云鲫8种单元型中确认了DNA复制终止相关的序列TAS、中央保守区序列(CSB-F、CSB-E和CSB-D)和保守序列CSB1,8种单元型含有3—5个TAS序列。在65个变异位点中,大部分序列变异为转换,8种单元型之间的序列差异在0.1%—6.3%之间。该研究为三倍体湘云鲫的繁殖和遗传改良提供了一些有价值的信息。更多还原

【Abstract】 The article presented the results of the first regular population study of triploid hybrids of crucian carp and common carp by sequencing the tRNA-Thr gene,tRNA-Pro gene,and partial control region of mitochondrial DNA(mtDNA).The 26 sequenced fragments ranged from 837 to 839 bp in length.The termination associated sequence(TAS) motifs,central conserved sequence blocks(CSB-F,CSB-E,CSB-D),and conserved sequence block(CSB1) were identified in the mtDNA control region.Most heteroplasmic fish had 3—5 TAS repeat units.The above sequences from 26 triploid hybrids were detected 65 polymorphic sites,yielding 8 haplotypes.Sequence divergence among different haplotypes ranged from 0.1% to 6.3%.This study provided some invaluable information for the reproducibility and genetic improvement of triploid hybrids.更多还原