【摘要】 采用两株真菌康宁木霉3.2774和白腐真菌5.776融合产生的融合菌株R201对秸秆进行液态发酵,发酵粗酶液经硫酸铵盐析,SephadexG-100柱层析2次后酶活可提纯8.89倍。经SDS-PAGE测得3种纤维素酶的分子量约为66.3、52.7ku和37.0ku。酶作用的最适温度为50℃,最适pH值为5.0,在40℃以下,pH值4～6之间酶活较稳定。浓度为5mmol/L的Fe2+对酶反应有明显促进作用。更多还原

【Abstract】 Cellulase was separated and purified from a culture filtrate of crasis R201 that was fused between Trichoderma koningii 3.2774 and Phanerochaete chrysosporium 5.776 through ammonium sulfate precipitation and twice Sephadex G-100 column chromatography. It was purified to 8.89 folds. The molecular weights of the three purified cellulase were about 66.3, 52.7ku and 37.0ku respectively. The optimum temperature and pH value for cellulose activity were 50℃ and 5.0, respectively.It was more stable below the temperature 40℃ and in the pH rang of 4～6. The enzymic reaction can be strongly activated by 5mmol/L Fe2+.更多还原