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胶原蛋白的分离纯化及氨基酸组成分析
Isolation and purification of collagen from porcine skin and analysis of amino acid composition
【摘要】 以新鲜猪皮为原料,用酶法提取胶原蛋白。对提取物用超滤、盐析等方法进行分离纯化,超滤方法较合适。通过电泳方法确定胃酶提取物中胶原蛋白的分子量分布范围在8~10ku,胰酶提取物中胶原蛋白的分子量分布范围在3~5ku。氨基酸分析表明胶原蛋白中Hyp含量达8.5%。
【Abstract】 The collagen of porcine skin was extracted by means of enzyme-digest method.The extract was purified further by ultrafiltration,malting out.The result showed that the method of ultrafiltration was fit.Polyacylamide gel electrophoresis(PAGE) indicated that collagen molecular weights were 8~10ku,3~5ku respectively in the extract obtained after the pepsin and trypsin hydrolysis.Amino acid composition of collagen indicated that the content of Hyp was 8.5%.
【关键词】 胶原蛋白;
羟脯氨酸;
分离;
纯化;
氨基酸组成;
【Key words】 collagen; hydroxyproline; isolation; purification; amino acid composition;
【Key words】 collagen; hydroxyproline; isolation; purification; amino acid composition;
- 【文献出处】 食品科技 ,Food Science and Technology , 编辑部邮箱 ,2007年10期
- 【分类号】TQ936
- 【被引频次】37
- 【下载频次】2069