【摘要】 在一定pH条件下,尼古丁和Cu²⁺能形成一定配位比的难溶于水的配合物.通过测定配合物或水溶液中Cu²⁺的含量,可在不用标准品的情况下测定尼古丁的含量.由于烟草浸出液所合成分比较复杂.其中的果胶、蛋白质等对Cu²⁺都有一定的吸附作用,因而直接从烟草浸出液中测定烟草尼古丁含量误差较大.通过平衡透析方法或预先对烟草浸出液进行除果胶、蛋白质等处理,然后测定,所得结果与行业标准（YC/T 8-93）分析结果对比误差较小,结果可靠.更多还原

【Abstract】 A method for determinating the nicotine in tobacco extract by flame atomic absorption（AAS） spectrometry is recommended as its convenience,quickness,low error and no nicotine standard substance have to be used.As the pro- rein and pectin etc.in tobacco extract can also absorb Cu²⁺,equilihrium dialysis or salting out was carried out in this method to ensure Cu²⁺ and nicotine can quantitatively be turned to coordination in order that nicotine can be quantified by the determination of Cu²⁺with flame atomic AAS.Several tobacco samples were determined by this method which prove to be low error comparing to the traditional method.更多还原