【摘要】 采用微流控装置结合电化学检测研究了测定人单个血红细胞中谷胱甘肽(GSH)的方法。在该方法中,细胞的进样、定位、溶膜以及细胞中谷胱甘肽的转移和检测都在配有通道端安培检测器的双T形芯片中完成。单个细胞用液压导入到双T的交界面,在电泳缓冲液中毛地黄皂苷的作用下,细胞膜被穿孔。再施加直流电压,细胞被溶膜。释放出来的GSH被此直流电压电迁移至通道端并在Au/Hg电极上被检测。用校正曲线法可以定量测定单个细胞中的GSH。更多还原

【Abstract】 A method for the determination of glutathione(GSH) in a single human hepatocarcinoma cell,using a microfluidic chip coupled with electrochemical detection,was developed.In this method,the cell injection,loading,and cytolysis,as well as the transportation and detection of intracellular GSH were integrated in a microfluidic chip with a doubme-T injector and an end-channel amperometric detector.A single cell was loaded in the double-T injector on the microfluidic chip,using liquid pressure.The docked cell was perforated in the electrophoretic buffer containing digitonin and was lysed under a direct current electric field.The GSH from the single cell was electrokinetically transported to the separation channel and was electrochemically detected at an Au/Hg electrode.The GSH in the single cell was quantitatively determined using a calibration curve.更多还原