【摘要】 研究了pH值和培养基主要组分对谷氨酸分析仪测定谷氨酸含量的影响,确定了γ-聚谷氨酸水解的最佳条件。结果表明,在pH=5～10内,溶液的pH值对谷氨酸分析仪的测定结果没有显著影响;模拟发酵液体系的试验,发酵液中各主要成分含量的变动范围为±25%时,对测定结果也没有明显影响。采用正交试验优化了γ-聚谷氨酸的水解条件。以2mL发酵液为例,其最佳水解条件为:4mL浓度为6mol/L浓盐酸,真空度为0.1MPa,110℃,24h。更多还原

【Abstract】 Effect of pH and fermentation broth components on the quantitative analysis of γ-Poly (glutamic acid) (γ-PGA) in its fermentation broth was performed on an glutamic acid analyzer. No obvious variations in analysis results were observed within a wide pH range,5.0 to 10.0. Similar stability was obtained with ±25 % fluctuations in the concentrations of major media components. Acid hydrolysis conditions such as temperature,vacuum,dose of HCl and reaction time were optimized by orthogonal experiments,in terms of their effect on the production of glutamic acid. The optimal operating conditions (as for a 2 mL fermentation broth) include: 4 mL 6 mol/L HCl,0.1 MPa vacuum,24 h reaction at 110 ℃.更多还原