【摘要】 用IPTG(诱导基因工程菌)M15/pQE31-gdh表达葡萄糖脱氢酶,纯化,该重组葡萄糖脱氢酶用于高纯度低聚果糖生产研究。以含量为51.11%(低聚果糖/总糖)低聚果糖的产品为底物,葡萄糖脱氢酶(5U/g总糖)作用2h,将低聚果糖含量提高到61.37%,消除了48.81%的葡萄糖。以30%(w/v)蔗糖为底物,利用有果糖基转移酶活性的米曲霉GX0015(15U/g总糖)作用6h后,加入葡萄糖脱氢酶(5U/g总糖)协同作用4h,得到成分为:低聚果糖含量76.87%、葡萄糖11.05%、蔗糖含量5.21%的产品。由果糖基转移酶和葡萄糖脱氢酶组成的系统能够有效降低葡萄糖含量,提高低聚果糖含量。更多还原

【Abstract】 Genetic Engineered M15/pQE31-gdh was induced with IPTG to express glucose dehydrogenase (GDH),and then was purified. The purified recombinant GDH was applied to research on high-content fructooligosaccharide(FOS)production. 61.37 % FOS (FOS/total sugar) was prepared after 48.81 % glucose in normal FOS product with 51.11 % FOS being removed by GDH(5 U/g total sugar).Mycelia of Aspergillus oryzae GX0015 with fructosyltransferase (FTase) activity and GDH were used to produce high-content FOS. After 30 %(w/v)sucrose solution was reacted for 6 h in the presence of F Tase (15 U/g total sugar) alone,GDH(5 U/g)was added,and 4 h later,76.87 % FOS with the remainder being 11.05 % glucose and 5.21 % sucrose on a dry weight basis was obtained. The system with both FTase and GDH was proved to be effective to raise FOS content and eliminate glucose.更多还原