【摘要】 本实验以我国来源丰富且价格低廉的玉米蛋白为原料,选用嗜热菌蛋白酶、胰凝乳蛋白酶、胰蛋白酶和碱性蛋白酶等4种酶来水解玉米蛋白制备降血压肽。选用马尿酰-组氨酰-亮氨酸(HHL)为血管紧张素转化酶(ACE)的模拟底物,利用反相高效液相色谱(RHPLC)检测ACE催化反应生成的马尿酸(Hip)的量,确定水解产物的ACE抑制活性从而来对酶进行筛选。研究结果表明,碱性蛋白酶的水解能力最强,碱性蛋白酶和嗜热菌蛋白酶的水解产物都达到了很好的ACE抑制活性(90%左右)。更多还原

【Abstract】 In this study, zein, a very abundant and cheap crop in China was hydrolyzed by thermolysin, chymotrypsin, trypsin and alkaline protease for processing antihypertensive peptides. Hippuryl-Histidyl-Leucine (HHL) was used as the simulant substrate of Angiotensin Converting Enzyme (ACE), and reverse-phase performance liquid chromatography (RHPLC) was used to measure the yield of Hippuryl (Hip). According to this process, the ACE inhibitory activity of each hydrolyzate was determined and chosen as the best protease. The results showed that alkaline protease has the optimum hydrolytic capability, while the hydrolyzate of alkaline and thermolysin shows great ACE inhibitory activity (up to 90%).更多还原