【摘要】 以Novo435固定化脂肪酶为催化剂,在无溶剂条件下催化甘油和辛酸合成单辛酸甘油酯。通过单因素试验确定加酶量、反应温度、反应时间三个因素的取值范围,并用响应面实验设计和分析方法对合成反应条件进行了优化。结果表明,在甘油辛酸摩尔比1:1、反应温度66.8℃、脂肪酶与反应底物质量比0.88%、无水的条件下反应11.5h,辛酸转化率达93.53%,单辛酸甘油酯含量为47.69%。更多还原

【Abstract】 Glycerol Monocaprylate was synthesized by glycerol and octanoic acid in a solvent free system, which was catalyzed by immobilized lipase-Novo435. The optimum conditions of synthesis were obtained through single factor test and response surface method, successively. When the ratio of glycerol to octanoic acid 1:1, the lipase dosage 0.88% (W/W) based on the reactant was employed in the reacting system, the conversion rate of octanoic acid could reach 93.53% at 66.8 ℃ for 11.5 h reaction. The content of glycerol monocaprylate was about 47.69% in the reacting production .更多还原