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粒毛盘菌培养条件及其产色素条件初步研究

Pilot Study on Culture Conditions of Lachnum and Its Producing Pigment Conditions

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【作者】 叶明谭炜陈辉赵婧唐传梅

【Author】 YE Ming,TAN Wei,CHEN Hui,ZHAO Jing,TANG Chuan-mei (College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)

【机构】 合肥工业大学生物与食品工程学院合肥工业大学生物与食品工程学院 安徽合肥230009安徽合肥230009

【摘要】 选择成熟的盘菌子实体进行组织分离,获得25株纯培养物。对其中的L.brasiliense84-2、L.sclerotii88-2、L.singerianum95-1培养条件进行初步研究,结果表明,这三株菌株生长的最适条件为:马铃薯250g/L,葡萄糖20g/L,蛋白胨5g/L,维生素B10.01g/L,25℃,pH5.5;对L.brasiliense84-2产色素条件进行研究,结果表明其产色素的最适条件为:马铃薯250g/L,NaHCO320g/L,KNO35g/L,甘氨酸0.01g/L,pH7.0。

【Abstract】 Tissue isolation performed on discomycetes specimens, 25 filament products were obtained. Culture conditions of L.brasiliense 84-2, L.sclerotii 88-2 and L.singerianum 95-1 were studied. The results showed that the optimum conditions for the growth of strains were potato 250 g/L, glucose 20 g/L, VB1 0.01 g/L, 25 ℃ and pH 5.5. The optimum conditions for producing pigment of L.brasiliense 84-2 were obtained as follows: potato 250 g/L, NaHCO3 20 g/L, KNO3 5 g/L, glycin 0.01 g/L and pH 7.0.

【关键词】 粒毛盘菌培养条件色素
【Key words】 Lachnumculture conditionspigment
【基金】 安徽省自然科学基金资助项目(050430502)
  • 【分类号】TS202.3
  • 【被引频次】22
  • 【下载频次】173
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