牦牛血中免疫球蛋白热变性动力学的研究

【摘要】 研究了免疫球蛋白的热稳定性及热变性动力学,结果表明:牦牛血IgG的热变性属于0级反应。在65、70、75、80、85℃条件下,IgG变性的D值分别为722.41、311.65、17.8、5.24、2.73、1.89min,在此温度范围内IgG变性的Z值为7.9℃,表观活化能为271.25kJ/mol。依据IgG热变性动力学方程计算,63℃30min杀菌,IgG变性率为2.79%;在72℃30s,75℃15s及85℃5s条件下杀菌,IgG变性率分别为0.58%、0.66%和3.03%,UHT处理(120℃4s)IgG活性全部丧失。这些结果说明,LTLT和HTST巴氏杀菌对牦牛血中IgG活性影响较小,但不可采用UHT工艺。更多还原

【Abstract】 Thermodynamic analysis indicated that the reaction class of heat denaturation of IgG in yak plasma in PBS buffer was 0.At 65,70,75,80,85℃,D values for IgG in yak plasma denaturation were 722.41,311.65,17.8,5.24,1.89min respectively.Within the temperature range,Z value for IgG in yak plasma denaturation was 7.9℃,apparent active energy was 271.25kJ/mol.LTLT and HTST pasteurization have little influence on IgG in yak plasma denaturation,but whole IgG activity was lost with UHT sterilization.更多还原