重金属离子对鲫蛋白ATPase活性和SH基总量的影响

【摘要】 研究了体外添加Pb2+、Cd2+、Cu2+等不同浓度的重金属离子对肌动球蛋白和肌球蛋白ATPase活性和SH基总量的影响。随Pb2+和Cd2+浓度的升高,肌动球蛋白的ATPase活性和SH基总量先升高,后下降,肌球蛋白的ATPase活性逐渐降低,SH基量先略有升高,后下降。Cu2+浓度的升高引起蛋白中ATPase活性和SH基含量明显下降,显示了较强的毒性。更多还原

【Abstract】 The effect of Pb2+,Cd2+ and Cu2+ with different concentration on ATPase activity and total sulfhydryl content of actomyosin and myosin was studied.The result showed that with the increasing of concentration of Pb2+ and Cd2+,ATPase activity and total sulfhydryl content of actomyosin raised slightly,and then decreased obviously.ATPase activity of myosin reduced,and total sulfhydryl content was similar to one of actomyosin.But the accession of concentration of Cu2+ reduced ATPase activity and total sulfhydryl content evidently,which displayed terribly toxicity.更多还原