【摘要】 以23株不同来源的酿酒酵母为研究对象,分别提取基因组DNA,试用50条随机引物对其进行随机扩增多态性DNA分析,筛选到两条具有菌株鉴别能力的随机引物。P09可以从2.412,ST—01,SK—26,ZD—01四株酿酒酵母基因组DNA中扩增出长度为433bp的Sc—433片断Sc—433;P46可以从2.1882,ST—01,NJ—02三株酿酒酵母基因组DNA中扩增出长度为665bp的Sc—665片断,其中仅有目的菌株ST—01能稳定的扩增出这2个标记。把这2个片断分别克隆到pUCmT质粒载体中,经过酶切鉴定后测序,根据序列设计特异性引物,把RAPD标记转化为特征区域序列扩增标记,为酿酒酵母菌株的分子鉴别提供了新的借鉴。更多还原

【Abstract】 In this paper,50 random primers were tested to the random amplified polymorphic DNA (RAPD)analysis on 23 strains of Saccharomyces cerevisiae isolated from different sources,and 2 effective primers named P09 and P46 were selected. Among the tested strains,4 strains(2.412,ST-01,SK-26,ZD- 01),have Sc-433 of 433bp fragment,were amplified with P09,and 3 strains(2.1882,ST-01,NJ-02),have Sc-665 of 665bp fragment,were amplified with P46,only the strain of ST-01 has the two markers simulta- neity.These two amplified DNA fragments were cloned into pUCmT plasmid and sequenced after identifica- tion of double digestion.Two pairs of specific primers were designed according to the sequence of amplified DNA fragments,and the sequenced characterized amplified region(SCAR)markers of ST—01 were devel- oped.These results could afford some new idea for molecular markers of Saccharomyces cerevisiae.更多还原