【摘要】 目的探讨联合检测抗环瓜氨酸肽(CCP)抗体和类风湿因子(RF)诊断类风湿关节炎(RA)的价值,并了解抗CCP抗体与RF之间的相关性。方法采用ELISA法和乳胶增强免疫比浊法,检测79例RA、52例非RA病人血清抗CCP抗体和RF。结果79例RA病人中单一抗CCP抗体阳性12例,单一RF阳性20例,两项同时阳性40例,同时阴性7例,一致率为59.5%(47/79)。52例非RA病人中单一抗CCP抗体阳性2例,单一RF阳性14例,无1例两项同时阳性。RA病人抗CCP抗体与RF阳性率显著高于非RA病人(χ2=49.713、30.666,P<0.01),RF阳性率高于抗CCP抗体,但差异无显著性(P>0.05);非RA病人RF阳性率显著高于抗CCP抗体(χ2=10.636,P<0.01)。抗CCP抗体诊断RA的特异度和阳性预测值分别为96.2%、96.3%,灵敏度为65.8%。抗CCP抗体和RF联合检测诊断RA的灵敏度、阴性预测值及准确度分别为91.1%、83.7%、82.4%。抗CCP抗体与RF无相关性(P>0.05)。结论抗CCP抗体诊断RA具有满意的特异度及很高的阳性预测值,对RA有较高诊断价值。抗CCP抗体与RF联合检测更有助于RA的临床诊断。更多还原

【Abstract】 Objective To evaluate the diagnostic value of serum anti-cyclic citrullinated peptide antibody(anti-CCP) and rheumatoid factor(RF) in patients with rheumatoid arthritis(RA). Methods ELISA and TPNIA techniques were used to detect serum levels of anti-CCP and RF in 79 patients with RA and 52 non-RA patients. Results Positive anti-CCP and RF were found in 40 patients with RA,while positive anti-CCP or RF alone was found in 12 or 20 with RA,respectively.Positive anti-CCP or RF alone was only noticed in two or 14 non-RA patients,respectively.Both anti-CCP and RF positive were not observed in non-RA patients.The positive rate of anti-CCP and RF of patients with RA was significantly higher than that of non-RA patients(χ2=49.713,30.666;P<0.01). Conclusion Determination of serum levels of anti-CCP by ELISA,with relatively high accuracy and specificity,is helpful in the diagnosis of patients with RA.更多还原