【摘要】 目的建立骨髓移植后植入状态监测的多重荧光标记短串联重复序列聚合酶链反应(STR-PCR)检测方法,为骨髓移植病人移植疗效评价及干预治疗提供可靠依据。方法于移植前、移植后7 d~3个月不同时间采集3例供受者静脉及骨髓血样,提取DNA作为模版,用多重荧光标记STR-PCR方法检测其基因型。结果2例供受者HLA-A、B、DRB1等6个等位基因完全相合病人骨髓移植后分别于第35天和3个月转为完全供者型,1例HLA半相合母子移植病人持续未植入,移植后第7、28天STR-PCR分析为受者基因型。结论荧光标记STR-PCR技术是骨髓移植后植入状态监测的有效方法,对移植效果有预测作用。更多还原

【Abstract】 Objective To establish a method of STR-PCR with fluorescence labelling system in order to monitor bone marrow transplantation. Methods Fluorescence labeling STR-PCR was established.DNA was extracted from peripheral blood and bone marrow nucleated cells and labeld with four color fluorescence before and from 7 days to 3 months after transplantation. Results Two patients converted into full donor chimeras.One patient received HLA haploidentical sibling donor stem cells failed to be engrafted. Conclusion Analysis of STR was a method of indication of engraftment and better than any other available methods.更多还原