【摘要】 为了探讨与中枢神经系统单胺类递质分泌失调有关疾病的中枢机制,人们对单胺类递质分泌动力学的研究越来越有兴趣。去甲肾上腺素是中枢神经系统重要的递质和调质,本文介绍了我们实验事最近发展的实时检测中枢神经系统去甲肾上腺素分泌的一些技术疗法,并比较了电化学微碳纤电极(carbon fiber electrode,CFE)测量与电生理、荧光显微测量技术优缺点,阐述了CFE技术在神经科学研究中的一个基本应用。更多还原

【Abstract】 In order to investigate the central nervous mechanism and the diseases involved in catecholamine transmitter secretion,the dynamics of catecholamine release is studied in single cell,brain slice or in vivo.Noradrenaline is an important neurotransmitter and modulator in the central nervous system（CNS）and the peripheral nervous system （PNS）.In the present paper,we first compared three real-time methods used to measure noradrenaline secretion in single cells（membrane capacitance,amperometry and confocal fluores- cence microscopy imaging）.Compared to the electrophysiological method and fluorescence microscopy,the basic usage of the carbon fiber electrode（CFE）in neuroscience research was presented as an example.Then,we presented a primary description of ion channels, including voltage-gated Na⁺,K⁺ and Ca²⁺channels in locus coeruleus（LC）neurons in rat brain slices.Finally,we presented example recordings of combined patch-clamp and amperometry measurements in LC neurons,indicating Ca²⁺-dependent quantal noradrenaline release following Ca²⁺influx through Ca²⁺channels.更多还原