【摘要】 目的探讨多重脑震荡(MCC)对大鼠认知行为的影响。方法成年SD大鼠24只,应用自制金属单摆式闭合性脑损伤机械打击装置复制MCC大鼠模型,随机分为对照组和MCC组,伤后应用Morris水迷宫实验,评价大鼠的空间学习记忆功能。结果在Morris水迷宫测试中,与对照组(8～14d测试结果分别为:64.74±23.43,33.16±17.70,36.59±27.53,37.60±18.20,37.14±26.03,16.85±10.63,10.02±6.51)相比,MCC大鼠伤后8～14d找到平台的时间明显延长,且在伤后的第9d(71.74±35.76)、第10d(63.14±31.02)、第13d(47.51±40.93)、第14d(42.39±43.33)差异具有显著性。在无平台探测实验中,与对照组(23.89±11.82)相比,MCC后第14d(15.12±6.56)大鼠在原有平台第三象限停留时间明显减少,差异显著(P<0.05)。结论实验性多重脑震荡大鼠在Morris水迷宫实验中,出现较为严重的空间认知行为障碍。更多还原

【Abstract】 Objective To study the effects of multiple cerebral concussion(MCC)on spatial cognitive behavior in rats.Methods Twenty-four Sprague-Dawley rats weighting between 280 g and 310 g were used.A MCC(one strike each day for continuous three days)rat model was produced using a simple pendulum device.One control and MCC groups were used at random(n=12 in each group).The Morris water maze(MWM)was used to access spatial learning and memory function.Results Compared with the control group,during the 8 th and 14 th day after MCC,the latency of finding the platform was much longer and there were significant differences in the 9 th,10 th,13 th,14 th day.Compared with the control group,probe trials demonstrated that the MCC produced reductions of staying time in the target quadrant,and there were statistically differences(P<0.05)in the MCC group.Conclusion Experimental MCC present severe cognitive deficits in rats after multiple cerebral concussion.更多还原