【摘要】 目的研究RASSF1A基因在膀胱移行细胞癌(TCC)中的表达及其临床意义。方法采用逆转录-聚合酶链反应检测45例膀胱TCC及其相应癌周正常组织中RASSF1A基因mRNA表达情况,采用免疫组织化学方法检测38例膀胱TCC及18例癌周正常组织中RASSFIA蛋白表达情况。结果与癌周正常组织相比,60.0%(27/45例)的膀胱TCC组织中发现RASSF1A mRNA表达异常下调。RASSF1A蛋白在膀胱TCC组织中的阳性表达率为36.8%,明显低于癌周正常组织中的83.3%(P<0.05)。不同临床分期和病理分级间RASSF1A mRNA表达异常和RASSF1A蛋白阳性表达的差异均无统计学意义(P值均>0.05)。结论RASSF1A的失表达可能参与膀胱TCC的发生。更多还原

【Abstract】 Objective To study the expression of RASSFIA gene in bladder transitional cell carcinoma (TCC)and its clinical significance.Methods The mRNA expression of RASSF1A in 45 TCC specimens and the corresponding adjacent normal tissues(n=45)were examined by reversed transcription PCR (RT-PCR).The protein expression of RASSF1A gene in bladder TCC tissues(n=38)and normal bladder tissues(n=18)were detected by immunohistochemistry method.Results The expression of RASSF1A mRNA was down-regulated in 60.0%(27/45)TCC specimens.The positive rates of RASSF1A protein in bladder TCC tissues were 36.8%, significantly lower than that of the normal bladder tissues (83.3%,P<0.05 ).The protein level in TCC were obviously lower than those in the normal tissues(P<0.05).Neither the protein level nor the mRNA expression of RASSF1A was correlated with the pathological grades and stages of TCC(both P>0.05).Conclusion The down-regulation of RASSF1A expression might contribute to the tumorigenesis of bladder cancer.(Shanghai Med J,2007,30:840-842)更多还原