【摘要】 目的寻找富锗金针菇粗多糖(CFVP)中发挥肝保护作用的有效成分。方法采用0.2%CCl4腹腔注射造成小鼠急性肝损伤模型,以富锗金针菇精品多糖FVP1、FVP2静脉给药,测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)的活力,肝组织匀浆,测定匀浆液中丙二醛(MDA)含量;采用二步分离法制备小鼠原代肝细胞,检测FVP2对原代小鼠肝细胞活力的影响,对CCl4损伤的原代肝细胞ALT活力的影响。结果FVP2中、高剂量组血清AST活力值,肝匀浆液MDA值,高剂量组血清ALT活力较模型组有显著性差异(P<0.05);FVP2能显著增加小鼠原代肝细胞的活力(P<0.01);可使原代肝细胞培养液中的ALT活性明显低于模型组(P<0.01)。结论FVP2可能是CFVP肝保护作用的主要有效成分。更多还原

【Abstract】 Purpose To find the active principle of crude Flammulina velutipes polysaccharides containing germanium with hepatoprotective effect.Methods The liver-impairment model mice were established by intra-abdominal injection of CCl4.The mice were treated with FVP1 and FVP2 by iv administration.After 5 days,the activity of serum ALT and AST and MDA was detected.The primary culture of murine monolayer hepatocytes was perfomed to see the effects of FVP2 on intracellular ALT.Results FVP2 at the dose of 2 mg/kg can significantly decrease the activity of serum ALT and can decrease the activity of serum AST and MDA at the dose of 0.4,2 mg/kg compared with the model control group(P<0.05).FVP2 can significantly increase the activity of primary hepatocytes and decrease the activity of intracellular ALT compared with model control group.Conclusion FVP2 is possibly the main constituent of hepatoprotective effect of CFVP.更多还原