【摘要】 用不同浓度TGF-1β对猪脐静脉血管内皮细胞(SUVEC)进行诱导,研究体外诱导SUVEC向平滑肌细胞分化的可能性。倒置显微镜下观察诱导前后细胞形态的变化,并采用免疫细胞化学SP法对细胞表面特异性标志物进行染色。结果表明,诱导后细胞失去内皮细胞典型的铺路石样形态,而呈现出肌成纤维样细胞;对诱导后的细胞进行免疫组化检测,发现5～10ng.mL-1 TGF-1β诱导后,平滑肌特异性蛋白-αSMA的相对表达量均显著升高;而浓度升高为20 ng.mL-1时,升高不显著;内皮细胞特异性标志(F-ⅧRAg)的表达均显著下降。该研究表明TGF-1β可诱导猪脐静脉血管内皮细胞向肌样细胞分化,但诱导分化的能力与TGF-1β的浓度有关。更多还原

【Abstract】 To demonstrate the possibility of swine umbilical vein vascular endothelial cells(SUVEC) to transdifferentiate into smooth muscle like cells,porcine vascular endothelial cells were incubated with transforming growth factor-β1 on different concentration.The changes in morphology or expression of specific marker of cells were analyzed by Immunohistochemical staining and image analysis method.It showed that expression of α-SMA increased distinctly when treated with 5～10 ng·mL-1 TGF-β1,whereas expression of F-Ⅷrelated antigen in the cells notably declined.Interestingly,20ng/mL TGF-β1 did not promote up-regulating of α-SMA.SUVECs lost their cobblestone morphology and converted into ragged cells in TGF-β1-treated culture.We demonstrated here that swine vascular endothelial cells can be transdifferentiated into smooth muscle like cells treated with TGF-β1.What’s more,the ability of transdifferentiation correlated to the concentration of TGF-β1.更多还原