【摘要】 目的:通过对大鼠实验性正畸牙移动过程中牙周组织内的核心结合因子α1(core binding factor α1,cbfα1)进行定性及定量分析,研究cbfα1在牙槽骨改建过程中与成骨细胞分化的相关性。方法:选用8周龄雄性成年Wistar大鼠42只,随机分为加力0、1、3、5、7、10、14d组,每组6只。分别在固定加力装置后0、1、3、5、7、10、14d时处死动物,制备标本,进行免疫组化染色。采用SPSS11.0软件包进行Dunnett检验,研究正畸牙移动过程中cbfα1的表达变化。结果:在正常大鼠的牙周组织中,cbfα1呈弱阳性表达。各加力组中cbfα1的阳性表达随时间变化呈先升高后回降的趋势,压力区的牙槽骨表面cbfα1阳性表达显著低于张力区牙槽骨表面(P<0.01)。结论:cbfα1参与了正畸牙移动的骨改建过程,与成骨细胞的分化密切相关,从而促进了牙周组织的改建和稳定。更多还原

【Abstract】 PURPOSE: To perform qualitative and quantitative analyses of core binding factor α1 (cbfα1) in periodontal tissue during the experimental rat tooth movement process and to investigate the active mechanism of cbfα1 in the osteoblast differentiation. METHODS: 42 male Wistar rats aged 8 weeks were divided into 7 groups (0,1,3,5,7,10 and 14 days groups) in random, 6 rats in each group. 42 rats were perfused and sacrificed at the 1st, 3rd, 5th, 7th, 10th and 14th day. All specimens were fixed, then proceeded with HE and cbfα1 immunohistochemical staining. The results were analyzed by SPSS11.0 software package for Dunnett test, and the histological changes of cbfα1 in periodontal tissue were evaluated. RESULTS: In the periodontal ligament(PDL) of non orthodontic treatment group, the expression of cbfα1 was lower; the expression of cbfα1 in every experimental groups increased firstly and decreased later; the expression of cbfα1 in the surface of the alveolar bone was lower in the compression side than in the tension side, and the number of positive cells was less too(P<0.01). CONCLUSION: cbfα1 participates in the process of bone remodeling during the process of orthodontic tooth movement, and it promotes the remodeling and stability of PDL.更多还原