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超声提取秦艽中龙胆苦苷的工艺优选
Optimization of the extraction technology of gentiopicroside from Gentiana macrophylla Pall by ultrasonic wave
【摘要】 目的:优选提取秦艽中龙胆苦苷的最佳工艺。方法:采用正交试验法,以龙胆苦苷含量为指标,考察了乙醇用量、提取时间、提取次数及提取温度对秦艽中龙胆苦苷提取率的影响,优选出合理的提取工艺。结果:以乙醇用量为30 ml、提取时间为20 min、提取次数为4次、提取温度为50℃最佳。结论:经验证性实验证明所优选条件可靠,适合秦艽中龙胆苦苷的提取工艺参考。
【Abstract】 Objective:to explore an optimum extration technology of gentiopicroside from Gentiana macrophylla Pall.Methods:using gentiopicroside as the index,the effects of alcohol amount,extraction time,extraction degree and extraction temperature on extraction technology were evaluated by orthogonal design method.Results:the superior technology of gentiopicroside was as follows:30 ml alcohol,20 minutes,extract 3 degrees and 50 ℃.Conclusion:the optimum extraction process is proved stable and accurate by verificated test,and it is suitable for extraction of gentiopicroside from Gentiana macrophylla Pall.It can be used for the theoretical basis.
【Key words】 Gentiana macrophylla Pall; gentiopicroside; ultrasonic wave extraction; orthogonal design;
- 【文献出处】 山东中医杂志 ,Shandong Journal of Traditional Chinese Medicine , 编辑部邮箱 ,2007年12期
- 【分类号】R284
- 【被引频次】3
- 【下载频次】230