【摘要】 目的分析急性白血病(AL)患者p16基因连锁的微卫星不稳定性(MSI)和杂和性缺失(LOH),了解p16基因改变与AL发生的关系。方法采用多重PCR方法检测53例AL患者骨髓及口腔黏膜细胞标本的p16基因连锁的3个微卫星位点(D9Sl62、D9S1748、D9S171),观察其MSI及LOH情况。结果53例AL患者中,MSI检出率为43.4%(23/53);位于9p21的p16基因连锁的微卫星D9Sl62、D9S1748、D9S171的LOH发生率分别为0(0/53)、5.7%(3/53)和9.4%(5/53),MSI发生率分别为13.2%(7/53)、7.6%(4/53)和7.6%(4/53)。结论AL患者p16基因连锁微卫星均可检测到高频率的MSI和LOH,说明p16基因突变与AL发生、发展有关。更多还原

【Abstract】 [Objective] To analyze p16 gene linked microsatellite instability(MST) and loss of heterozygosity(LOH) of acute leukemia(AL) and the relation between p16 gene change and AL.[Methods] 3 microsatellite sites(D9Sl62,D9S1748,D9S171)linked to p16 gene were detected by multiplex-PCR in mouth cavity mucosa and bone marrow samples from 53 leukemia patients.MSI and LOH were obversed.[Results] The rate of MSI in 53 AL patients was 43.4%(23/53).In p16-linked microsatellite D9SI62,D9SI748 and D9SI71 on 9p21,frequencies of LOH was 0(0/53),5.7 %(3/53) and 9.4%(5/53) respectively,frequencies of MSI were 13.2%(7/53),7.6%(4/53) and 7.6%(4/53) respectively.[Conclusion] High frequency of MSI and LOH can be found in p16 gene-linked microsatellites in AL,it hints that p16 gene mutation has relation with genesis and development of AL.更多还原