【摘要】 目的探讨乳腺癌转移抑制基因(breast cancer metastasis suppressor 1,BRMS1)在声门上型喉鳞状细胞癌组织中的表达及其临床意义。方法免疫组化法检测60例原发性声门上型喉癌组织(肿瘤组)和其相邻的癌旁正常喉黏膜(对照组)石蜡标本组织细胞中BRMS1蛋白的表达。结果对照组均有较强的BRMS1蛋白的表达,肿瘤组阳性表达率为35.0%(21/60),且均为低表达,两者之间差异有统计学意义(P<0.05)。BRMS1蛋白在病理高分化标本中阳性表达率为43.6%(17/39),在中、低分化标本阳性表达率19.0%(4/21),两者之间差异有统计学意义(P<0.05);在临床Ⅱ期标本阳性表达率为72.7%(8/11),临床Ⅲ+Ⅳ期标本阳性表达率26.5%(13/49),两者之间差异有统计学意义(P<0.05);颈部淋巴结N0组标本阳性表达率72.7%(16/22),颈部淋巴结N+组阳性率13.7%(5/38),两者之间差异有统计学意义(P<0.05)。BRMS1蛋白阳性组患者与BRMS1蛋白阴性组患者的术后生存率无统计学差异(P>0.05)。结论BRMS1蛋白在声门上型喉癌中低表达或不表达,且其表达可能与声门上型喉癌的临床分期、病理分化以及颈部淋巴结转移有关。更多还原

【Abstract】 Objective To investigate the expression of breast cancer metastasis suppressor 1(BRMS1)protein in supra-glottic cancer and to evaluate its clinical significance.Methods The expression of BRMS1 protein was determined by using the immunohistochemical method in 60 cases of paraffin-embeded supraglottic cancer tissues and their surrounding laryngeal normal mucosa tissues(LNT).Results The expression of BRMS1 protein was positive in 35.0%(21/60) cases of carcinoma and 100%(60/60) in LNT,and it was lower in supra-glottic cancer than in normal mucosa,also it was significantly different between supra-glottic cancer tissues and the LNT(P<0.05).The expression of BRMS1 protein in supra-glottic carcinoma was related to the histological grade(P<0.05), that in a high cell differentiation was 43.6%(17/39) and in a moderate and a low cell differentiation was 19.0%(4/21),and it was related to the clinical stage that in stage Ⅱ was 72.7%(8/11)and in stage Ⅲ-Ⅳ was 26.5%(13/49),also it was related to cervical lymphatic metastasis(P<0.05),that with cervical lymphatic metastasis was 13.7%(5/38) and without cervical lymphatic metastasiswas 72.7%(16/22).The 3 and 5-year survival was not significantly different between the positive and the negative groups.Conclusion Expression of BRMS1 protein in supra-glottic cancer is lower than that in normal mucosa.The decrease of BRMS1 protein expression may be related to the clinical stage,low differentiation and cervical lymph node metastasis of supra-glottic cancer.更多还原