【摘要】 目的检测cyclin E在胆管癌中的表达,并结合多种临床因素探讨其与肿瘤神经浸润及预后的关系。方法应用S-P免疫组化技术,检测35例胆管癌、25例癌旁正常胆管组织、11例胆总管良性病变组织中cyclin E的表达,并对可能影响预后的因素进行统计学分析。结果cyclin E在胆管癌组织中表达的阳性率高于癌旁正常胆总管组织(P<0.01)和胆总管良性病变组织(P>0.05);cyclin E表达与肿瘤神经侵犯密切相关(P<0.01),而与肿瘤大小、分化程度、周围组织浸润及TNM分期无相关性(P>0.05);cyclin E阴性组的生存率明显长于阳性组(P<0.01)。结论cyclin E在胆管癌中的表达与肿瘤神经侵犯密切相关,cyclinE可作为判断胆管癌预后的指标之一。更多还原

【Abstract】 Objective To explore the expression of cyclin E in cholangiocarcinoma and investigate the relevance of cyclin E and several clinical factors with infiltration of nerve and prognosis.Methods The S-P immunohistochemical staining method was used to determine the expression of cyclin E in 35 cases of cholangiocarcinoma tissues,25 cases of normal bile duct tissues beside the tumor and 11 cases of bile duct′s benign disease.Results The positive expression rate of cyclin E in cholangiocarcinoma was higher than that in the infiltration of the surroundings(P<0.01) and tissues of the bile duct′s benign diseases(P>0.05).The positive expression of cyclin E was related to the infiltration of nerves(P<0.01),but was not related to the size of the tumor,pathologyic stage,infiltration or clinical TNM stage(P>0.05).The median survival time was significantly longer in patients negative for the cyclin E expression than that in those positive for the expression(P<0.01).Conclusions The positive expression of cyclin E is related to infiltration of nerve.Cyclin E may be one of markers to evaluate the prognosis of cholangiocarcinoma.更多还原