【摘要】 目的进一步观察谷氨酰胺(Glutamine,Gin)对过度训练大鼠肠粘膜屏障功能的保护作用并探讨其可能的作用机制。方法:120只健康雄性SD大鼠随机分为普通训练组(C组)(n=30)(作为对照组)、补充Gln+普通训练组(CG组)(n=30)、过度训练组(O组)(n=30)和补充Gln+过度训练组(OG组)(n=30)。采用模拟过度训练大鼠模型,应用形态学手段结合免疫组化和放射免疫技术,观测肠道形态、固有层IgA浆细胞、粘液IgA、Ⅱ型磷脂酶A2(Ⅱ型PLA2)和溶菌酶分泌等指标的变化情况。结果Gln能明显减轻过度训练大鼠肠粘膜结构损伤。OG组IgA浆细胞计数及其平均吸光度、粘液IA含量、Ⅱ型PLA2水平和溶菌酶含量与O组比较,显著增加(P<0.01)。结论Gln可能通过刺激大鼠小肠肠壁分泌Ⅱ型PLA2和溶菌酶的作用来维护过度训练大鼠肠粘膜屏障功能。更多还原

【Abstract】 Objective To investigate the protective effects of glutamine on intestinal mucosal barrier in overtraining rat model and explore its possible mechanisms. Methods 120 healthy male SD rats were randomly divided into the control group(group C)(n=30), Gln+control group(group CG) (n=30), overtraining group(group O) (n=30), Gin +overtraining group (group 0(3) (n=30). Adopted overtraining rat model, the histological appearance of intestine, mucosal IgA immunity and its mechanisms type II phospholipase A2(type II PLA2),lysozyme were measured by means of the electron microscope technique, radioimmunoassay, immunohistochemical and image analysis and other corresponding methods respectively. Results Glutamine could significantly attenuate intestinal mucosal injuries and ameliorate bacteria translocation, the number of IgA-containing cells and their mean optical density(A) and the level of mucus/IgA and the type II PLA2 activity and lysozyme content of intestinal mucosa were all significantly decreased in group OG compare with group O. Conclusion The possible mechanisms might involve the Gin can significant stimulate the small intestine of mice to excrete type I1PLA2 and lysozyme and resulting in the increase of type II PLA2 and lysozyme contents in the intestinal tract and enhancing the function of mucosal barrier of intestine.更多还原