【摘要】 根据已报道的部分哺乳动物线粒体细胞色素C氧化酶(Cytochrome C Oxidase)亚基Ⅲ基因(mtCOXⅢ)的相关信息设计引物,运用PCR技术,首次从亚洲黑熊四川亚种(以下简称四川黑熊,Ursus thibetanus mupinensis)的肌肉组织总DNA中成功克隆了mtCOXⅢ基因的编码序列,并对其进行了初步分析.结果表明:四川黑熊mtCOXⅢ基因序列全长784 bp,ORF是783 bp,编码261个氨基酸的蛋白质,该蛋白的等电点为6.47,分子量为29840.6 Da.四川黑熊的mtCOXⅢ与已报道的部分哺乳动物的mtCOXⅢ基因具有很高的同源性.进一步分析发现,四川黑熊线粒体细胞色素C氧化酶亚基Ⅲ功能位点的位置、种类和数量均与已报道的部分哺乳动物具有很高的相似性.更多还原

【Abstract】 Mitochondrial Cytochrome C Oxidase subunit Ⅲ gene(mtCOXⅢ) in muscular tissue of Asiatic black bear Sichuan subspecies(Ursus thibetanus mupinensis) was amplified for the first time using PCR technology with primers designed according to mtCOXⅢ genes of some mammals reported,which was cloned,sequenced and analyzed preliminarily.The mtCOXⅢ sequence of Asiatic black bear Sichuan subspecies is 784 bp containing ORF 783 bp and codes 261 amino acid residues whose isoelectric point is 6.47 and molecular weight is 29840.6 Da.The mtCOXⅢ gene is highly homologous to some mammals reported.Further analysis indicates that the position,the type and the quantity of the function sites of cloned mitochondrial Cytochrome C Oxidase subunit Ⅲ are almost same to some mammals reported.更多还原