【摘要】 目的:研究5-LOX/COX-2抑制剂DHDMBF30对人胰腺癌Capan-2细胞增殖和凋亡的影响。方法:RT-PCR、MTT、FCM和电镜检测DHDMBF30对胰腺癌Capan-2细胞的增殖和凋亡的影响。结果:DHD-MBF30明显抑制Capan-2细胞增殖,IC50(50%的细胞失去活性时所需要的药物浓度)为12μmol/L,减弱5-LOX、COX-2和VEGF的表达。Capan-2经DHDMBF30处理后凋亡峰明显升高(27.67±0.52)%,P<0.001。结论:DHDMBF30能够抑制胰腺癌Capan-2细胞的增殖,诱导其凋亡。更多还原

【Abstract】 OBJECTIVE:To study the effect of 5-LOX/COX-2 inhibitor DHDMBF30 on proliferation and apoptosis of pancreatic cancer cell line Capan-2.METHODS:The effect of 5-LOX/COX-2 inhibitor DHDMBF30 on proliferation and apoptosis of pancreatic cancer cell line Capan2 was investigated by RT-PCR,MTT assay,FCM and an electron microscope.RESULTS:DHDMBF30 inhibited the proliferation of cell line Capan-2 and reduced the expressions of 5-LOX,COX-2 and VEGF.IC50was 12 μmol/L.After cell line Capan-2 was treated with DHDMBF30,it was found that the apoptosis peak of the experimental group was significantly higher than that of control group,P<0.001.CONCLUSION:DHDMBF30 can inhibit the proliferation of cell line Capan-2 and induce its apoptosis.更多还原