【摘要】 目的探讨汉防己甲素对氟康唑的体外增效作用。方法参照美国国家实验室标准化委员会推荐的M27-A和M44-A方案,测定氟康唑和/或汉防己甲素作用于16株同一亲本来源白念珠菌时的最低抑菌浓度(MIC);绘制氟康唑和/或汉防己甲素作用于白念珠菌时的杀菌曲线。结果氟康唑与汉防己甲素两药联合使用较氟康唑(1MIC)单独使用时菌落计数明显减少,且在菌株CA1、CA2,CA3～CA15,CA16、CA17三个组间表现不同,差异有统计学意义(P<0.05)。结论汉防己甲素对氟康唑有增效作用。更多还原

【Abstract】 Objective The synergism effect of tetrandrine to Fluconazole when it associated with Fluconazole against to Candida albicans in vitro.Methods Referring to M27-A method for broth dilution antifungal susceptibility testing of yeasts and M44-A method for antifungal disk diffusion susceptibility testing of yeasts recommended by US National Committee for Clinical Laboratory Standards to do broth micro-dilution antifungal susceptibility testing and agar disk diffusion testing.To measure the minimum inhibitory concentrations of strain 16 isolates of Candida albicans from a single strain when they are against by Fluconazole or tetrandrine alone or Fluconazole association with tetrandrine.To design the kill-time curve of Fluconazole or tetrandrine alone or Fluconazole association with tetrandrine against to Candida albicans.Results The MIC obviously decreased when Fluconazole associated with tetrandrine in 40μg/ml against to Candida albicans in the broth micro-dilution antifungal susceptibility testing.The counting of colonies obviously decrease when Fluconazole in 1MIC association with tetrandrine against to Candida albicans,and the CA1、CA2,CA3～CA15,CA16 and CA17 were different and show a significant difference(P<0.05).Conclusion Tetrandrine has synergism effect to Fluconazole.更多还原