【摘要】 目的探讨NF-kB/IκB-α信号通路对全反式维甲酸(ATRA)诱导HL-60细胞凋亡的影响。方法将HL-60细胞与不同浓度的ATRA共同孵育,MTT法观察ATRA对细胞增殖作用,流式细胞术及电镜分析细胞凋亡,Western-Blot检测NF-κB及IκB-α的变化。结果MTT显示ATRA呈时间-剂量依赖方式抗HL-60细胞增殖,作用72h流式细胞术DNA直方图上出现典型的亚二倍体凋亡峰,透射电镜下见细胞核染色质致密浓缩,凋亡小体形成,Western-Blot产物显示HL-60细胞NF-κBp65下降,而IκB-α上升。结论ATRA能抗HL-60细胞增殖诱导凋亡,该过程可能与抑制IκB-α的降解阻止NF-κB的活化有关。更多还原

【Abstract】 Objective To explore effect of NF-κB/ IκB-α signal pathway on apoptosis of HL-60 cells induced by All-trans retinoic acid.Methods HL-60 cells were cultured with ATRA in different concentrations.Cell viability of proliferation was assessed by MTT assay. Western-blot was used to analyse the expression of NF-κB and IκB-αof HL-60 cells. Apoptosis were also observed by flow cytometry and transmission electron microscope. Results Proliferation of HL-60 cells was inhibited significantly by ATRA in a time dependency and dosage-dependant manner. After cultured with ATRA for 72h, HL-60 cells were induced to apoptosis. Transmission electron microscopy found a typical apoptosis.There was an apoptosis peak by FCM. The level of IκB-α was up-regulated after ATRA stimulation, while NF-κB was down-regulated. Conclusion ATRA can induce apoptosis of HL-60 cells,and this effect involves the activation of IκB-α and the degradation of NF-κB.更多还原