【摘要】 构建了果实特异性启动子驱动的含编码丙型肝炎病毒包膜蛋白E2基因的植物表达载体,为研制有效的转基因植物丙肝疫苗打下基础。以载体pCAMBIA2300为骨架引入pBIl21中的GUS基因,用目的片段E2基因替换其中的GUS基因构建了pCAM-BIA2300G-E2载体,将番茄的果实特异性启动子E8引入pCAMBIA2300G-E2载体中构建了pE8-E2植物表达载体。用限制性内切酶消化重组载体,结果表明重组载体都含有所插入的目的片段。果实特异性启动子驱动的含E2基因的植物表达载体的成功构建,为抗丙型肝炎病毒的口服疫苗研究提供了试验基础。更多还原

【Abstract】 To construct a plant effective expression vector driven by a fruit-specific promoter for the expression of hepatitis C virus E2 gene,and prepare for the development of an effective against hepatitis C vaccine,the following work was done.A simple and practical middle vector,pCAMBIA230G,was constructed by using pCAMBIA2300 which was inserted with GUS gene from the pBI121.The vector,pCAMBIA2300G-E2,was constructed by replacing GUS gene with HCV E2 gene.The tomato fruit-specific promoter gene E8 was introduced to pCAMBIA2300G-E2 to form pE8-E2.Digestion with restriction enzymes proved that all recombinant vectors had the inserts with expected length of the target fragments.Plant expression vector containing HCV E2 gene driven by fruit-specific promoter was successfully constructed,which may provide a useful experiment foundation for further studying edible vaccine against HCV.更多还原