【摘要】 目的:采用蛋白质组学技术鉴定肝癌细胞株HepG2的G2/M期细胞内的热激蛋白。方法:提取肝癌细胞株HepG2的G2/M期细胞的蛋白质,采用双向电泳的方法分离蛋白质,应用图像扫描仪及质谱(MALDI-TOF-MS)分析鉴定HepG2的G2/M期细胞表达的蛋白质。结果:采用双向电泳技术分离,并用质谱成功鉴定出HepG2的G2/M期细胞的热激蛋白。结论:肝癌细胞株HepG2的G2/M期细胞内的热激蛋白的成功鉴定,为探讨HSP在细胞生长和增殖中的作用奠定了基础,并为临床上肿瘤的治疗提供参考依据。更多还原

【Abstract】 Objective:To identify heat shock protein in G2/M phase from HepG2 using proteomics technique.Methods:Proteins of G2/M phase were prepared from human hepatocarcinoma cells and separated using technique of two-dimensional electrophoresis,then analysed and identified via imagine scanner and MALDI-TOF-MS.Results:Heat shock protein in G2/M phase from HepG2 was successfully separated and identified using technique of two-dimensional electrophoresis and MALDI-TOF-MS.Conclusion:The identification of heat shock protein in G2/M phase from HepG2 is laid the foundation of research on HSP in the cell growth and proliferation.It also provides the basis for the treatment of tumor.更多还原