【摘要】 利用间接免疫蒌光标记技术研究 Ser10磷酸化的组蛋白 H3和微管蛋白在小麦根尖细胞中有丝分裂过程中的动态分布情况,结果显示在小麦根尖细胞有丝分裂过程中 Ser10磷酸化的组蛋白 H3的出现和消失与染色体的凝集和解凝集的过程存在时空上的相关性,在有丝分裂的过程中这种蛋白在着经线粒上的定位有有助于染色体向两极移动,研究结果还表明,在有丝分裂过程中,微管蛋白发生了重组,成束的垂直排列在赤道板的两侧,协助细胞有丝分裂过程的顺利完成。更多还原

【Abstract】 The indirect immunofluorescence labeling technology was used to study the dynamic distribution of Ser-10 phosphorylated histone H3 and microtubules in wheat root ceils during the mitosis.The result showed that during mitotic division,the Ser-10 phosphorylated histone H3 start from the prophase and vanished at telophase,remaining mainly in the pericentromeric regions at metaphase and anaphase.During the mitosis the microtubules take on regulated change pattern.May be during the mitosis the Ser-10 phosphorylated histone H3 is the component of the centromere/kinetochore and responsible for interact with microtubules and assist the chromosome movement.更多还原