【摘要】 目的:体外培养大鼠骨髓间充质干细胞(MSCs),探讨其对移植肾诱导免疫耐受作用。方法:无菌条件下取大鼠股骨、胫骨,用直接贴壁法分离纯化MSCs,体外扩增,在注射MSCs的基础上,行同种异体肾移植,实验大鼠分为注射大鼠骨髓MSCs肾移植组(I组)和单纯肾移植组(Ⅱ组),均未用任何免疫抑制剂,术后3d结扎受体右肾动脉(左侧为移植肾);观察右肾动脉结扎术后受体存活时间和右肾术后移植肾的病理变化。结果:I组大鼠生存期(15.50±6.35)d较Ⅱ组(6.50±3.ll)d明显延长,且前者移植肾形态学观察和功能状态均明显优于后者。结论:MSCs在体外很容易分离培养和扩增,MSCs对早期移植肾具有保护作用。更多还原

【Abstract】 Objective:To cultivate rat MSCs and explore the protective effects of MSCs on the transplanted kidney.Methods:Bone marrow was collected from SD rat by flushing femurs and tibias under sterile condition.MSCs were purified by stillness-stick cultivation in vitro.Forty wistar rats were transplanted with the left kidneys of SD rats,twenty wistar rats were infused with MSCs(Group I),not did the other twenty Wister rats(GroupⅡ).At day3,the right renal pedicle of recipients were ligated.The survival time and morphology of the transplanted kidneys were observed.Results:The survival time of Group I(15.50 ± 6.35)d was longer than that of Group Ⅱ[(6.50 ± 3.11)d,P < 0.05].The former transplanted kidney morphology observation and function obviously surpass the latter.Conclusion:MSCs can be isolated,cultured and expanded easily in vitro,and have protective effect on the transplanted kidney in early period.更多还原