【摘要】 酶法拆分(±)-N-(2,6-二甲苯基)-丙氨酸甲酯,其反应体系为:在含1 mmol(±)-N-(2,6-二甲苯基)-丙氨酸甲酯的100 mL 0.2 mol.L-1磷酸缓冲液(PBS)中,添加2 g聚乙二醇(PEG-6000),并用皱落假丝酵母脂肪酶(Candida rugosalipases,CRL)拆分,反应后分离得到R-(+)-N-(2,6-二甲苯基)-丙氨酸甲酯。进一步采用高效液相色谱检测酶促反应的转化率,采用250 mm×4.6 mm,5μHypersil(ODS色谱柱,以甲醇与水混合液(体积比为80∶20)为流动相,230 nm为检测波长,外标法峰面积定量。(±)-N-(2,6-二甲苯基)-丙氨酸和其甲酯的回收率在91.5%~96.1%之间。更多还原

【Abstract】 An enzymatic method for the production of methyl R-(+)-N-(2,6-dimethylphenyl)alanine has been developed.The process was catalyzed by Candida rugosa lipases(CRL),which contained 1 mmol of methyl(±)-N-(2,6-dimethylphenyl)alanine,100 mL of 0.2 mol·L-1 phosphate buffer solution(PBS),as well as 2 g of polyethylene glycol(PEG-6000),methyl R-(+)-N-(2,6-dimethylphenyl)alanine was separated from the reaction.The concentration of(±)-N-(2,6dimethylphenyl)alanine and its methyl ester were determined with high-performance liquid chromatography on a 250 mm×4.6 mm,5 μHypersil ODS column using methanol+water(V∶V=80∶20)as the mobile phase,UV detection at 230 nm,and an external standard allowing quantification by measurement of peak area.The recovery was 91.5%-96.1%.The method was also used to determine the conversion of the enzyme-catalyzed reaction.更多还原