【摘要】 利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因(MuERV-L)的表达进行研究,并通过appd icolin(DNA复制抑制剂)、TSA(组蛋白去乙酰化酶特异性抑制剂)分别抑制1-细胞期DNA的复制和合子基因组激活(ZGA)过程中组蛋白去乙酰化,探索MuERV-L基因表达的变化。结果表明:MuERV-L基因的转录在受精后9~11 h快速启动,并在2-细胞阶段出现瞬时高表达。抑制试验显示:1-细胞期DNA的复制受抑制后,MuERV-L基因的转录也被显著抑制(P<0.05);在TSA处理的4-细胞胚胎中,MuERV-L基因显著高表达(P<0.01),且其水平达到正常2-细胞的转录水平。提示:该基因可能对小鼠胚胎ZGA过程起重要作用。更多还原

【Abstract】 The pattern of murine endogenous retrovirus-like gene(MuERV-L)mRNA expression was examined in the early preimplantation development of mice,using real-time RT-PCR technique.Effect of DNA replication and histone deacetylation on the zygotic genome activation(ZGA)of mouse in vitro fertilization(IVF)embryos was also investigated using 1-cell embryos treated with appidicolin and TSA,which inhibit DNA replication and the activities of histone deacetylase,respectively.The results indicated that transcription of the MuERV-L gene was rapidly activated from 9 to 11 h after IVF,then transiently increased during the 2-cell stage.In the inhibition experiment,MuERV-L gene transcription was downregulated significantly following DNA replication repressed in the 1-cell stage.Moreover,high expression of MuERV-L mRNA was observed in 4-cell embryos treated with TSA,and the transcriptional level of MuERV-L gene in 4-cell embryos achieved the same that in the normal 2-cell embryos.Conclusion:MuERV-L plays an important role in the ZGA of mouse embryos.更多还原