【摘要】 建立了同时测定金胆片中龙胆苦苷、白藜芦醇及其苷含量的HPLC方法,以求用于中成药的质量监测。采用梯度洗脱,色谱柱为μBondapakTMC18(3.9 mm×300 mm,10μm)柱,流动相为水-甲醇,流速为1.0 mL.min-1,检测波长为288 nm,0.02 AUFS,样品采用乙醇-水(85:15,v/v)超声提取。龙胆苦苷线性范围1.877~75.06 mg.L-1,r=0.999 9;白藜芦醇苷的线性范围6.432~257.28mg.L-1,r=0.999 9;白藜芦醇的线性范围0.680~27.19 mg.L-1,r=0.999 9;平均加标回收率分别为:100.78%,RSD=3.61%;104.67%,RSD=3.29%;101.48%,RSD=3.68%;本方法灵敏度高、选择性好、操作简便、费用低,适合于该类中成药的有效成分快速分离分析。更多还原

【Abstract】 A HPLC method for simultaneous determination of gentianal,polydatin and resveratrol in jindan tablets was established.A gradient programmed method was employed on a μBondapakTM C18(3.9 mm×300 mm,10 μm) by using water-methanol as mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength was at 288 nm with 0.02 AUFS.The samples were extracted with ethanol-water(85:15,v/v) by ultrasonic treatment.The linear ranges of gentianal,polydatin and resveratrol are 1.877~75.06 mg·L-1,6.432~257.28 mg·L-1 and 0.680~27.19 mg·L-1,respectively.Their average recoveries are 100.78%,104.67% and 101.48%(RSD=3.61%,3.29%,3.68%.n=5),respectively.This method has high sensitivity,good selectivity,simplicity and practicality,which is a fast analytical method for the quality evaluation and routine supervision of Chinese Traditional Medicines Jindan tablet.更多还原