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37例性病门诊患者泌尿生殖道沙眼衣原体基因分型研究

Genotyping of 37 Chlamydia Trachomatis Strains from the Patients of STIs Clinic

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【作者】 李燕娃潘锐光江海燕黄西元潘洪亮郑和平吴彩威

【Author】 LI Yan-wa PAN Rui-guang JIANG Hai-yan,et al (Foshan Institute of Dermatology, Foshang, Guangdong 528000, China)

【机构】 广东省佛山市皮肤病防治所广东省皮肤性病防治中心广东省佛山市三水区疾病防治所

【摘要】 目的:了解性病门诊高危人群中沙眼衣原体分型特征。方法:采用巢式PCR扩增omp 1基因的VS1-VS2序列,自动测序仪测序,对沙眼衣原体临床菌株分型。结果:以12株标准株omp 1基因VS1-VS2序列为标准,37例沙眼衣原体感染样本基因分型共检出9个血清型,B型1株(2.7%)、D型5株(13.5%)、E型11株(29.7%)、F型5株(13.5%)、G型1株(2.7%)、H型3株(8.1%)、J型9株(24.3%)、K型1株(2.7%)、混合感染型1例(2.7%)。结论:本组泌尿生殖道沙眼衣原体感染的基因型以E型、J型、F型、D型为主,分型对于监测特定型别的流行动态有重要意义。

【Abstract】 Objective:To understand the genotype distribution of Chlamydia trachomatis in Pa- tients from STIs Clinic.Methods:VS1-VS2 of ompl gene was amplified by nest PCR and se- quenced by a sequencer.Identity of VS1 and VS2 were analyzed and genotyped using DNAstar soft- ware.Results:9 serotypes were identified among the 37 chlamydia strains,one stain was type B (2.7%),5 strains were type D(13.5%),11 stains were type E(29.7%),5 strains were type F (13.5%),1 strain was type G(2.7%),3 strains were type H(8.1%),9 strains were type J (24.3 %),1 stain was type K (2.7 %).Conclusion:The most prevalent types in Fushan were type F,E,J and D.Study on the molecular epidemiology of C.trachomatis infection is important for controlling the transmission of special genotypes.

【关键词】 沙眼衣原体测序分型
【Key words】 Chlamydia trachomatisSequenceGenotype
  • 【文献出处】 岭南皮肤性病科杂志 ,Southern China Journal of Dermato-Venereology , 编辑部邮箱 ,2007年06期
  • 【分类号】R759
  • 【被引频次】1
  • 【下载频次】53
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