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37例性病门诊患者泌尿生殖道沙眼衣原体基因分型研究
Genotyping of 37 Chlamydia Trachomatis Strains from the Patients of STIs Clinic
【摘要】 目的:了解性病门诊高危人群中沙眼衣原体分型特征。方法:采用巢式PCR扩增omp 1基因的VS1-VS2序列,自动测序仪测序,对沙眼衣原体临床菌株分型。结果:以12株标准株omp 1基因VS1-VS2序列为标准,37例沙眼衣原体感染样本基因分型共检出9个血清型,B型1株(2.7%)、D型5株(13.5%)、E型11株(29.7%)、F型5株(13.5%)、G型1株(2.7%)、H型3株(8.1%)、J型9株(24.3%)、K型1株(2.7%)、混合感染型1例(2.7%)。结论:本组泌尿生殖道沙眼衣原体感染的基因型以E型、J型、F型、D型为主,分型对于监测特定型别的流行动态有重要意义。
【Abstract】 Objective:To understand the genotype distribution of Chlamydia trachomatis in Pa- tients from STIs Clinic.Methods:VS1-VS2 of ompl gene was amplified by nest PCR and se- quenced by a sequencer.Identity of VS1 and VS2 were analyzed and genotyped using DNAstar soft- ware.Results:9 serotypes were identified among the 37 chlamydia strains,one stain was type B (2.7%),5 strains were type D(13.5%),11 stains were type E(29.7%),5 strains were type F (13.5%),1 strain was type G(2.7%),3 strains were type H(8.1%),9 strains were type J (24.3 %),1 stain was type K (2.7 %).Conclusion:The most prevalent types in Fushan were type F,E,J and D.Study on the molecular epidemiology of C.trachomatis infection is important for controlling the transmission of special genotypes.
- 【文献出处】 岭南皮肤性病科杂志 ,Southern China Journal of Dermato-Venereology , 编辑部邮箱 ,2007年06期
- 【分类号】R759
- 【被引频次】1
- 【下载频次】53